KLRG1 blockade increases ser⁴⁷³ Akt phosphorylation. (A) Representative immunoblots of pser⁴⁷³ and total Akt for CD8⁺ CD28/CD27 subsets after E-cadherin blockade. (B) Densitometric analysis of one Western blot showing the ratio between pSer Akt/total Akt after E-cadherin blockade in CD28/CD27 subsets. (C) Representative histograms of pser⁴⁷³ Akt flow cytometry in the CD8⁺ CD27/28 populations with or without E-cadherin. (D) Pooled data showing the change in pser⁴⁷³ Akt phosphorylation levels in donors after E-cadherin blockade. Graphs show the mean ± SE for 4 donors.