American Society of Hematology

Figure 1

$CD45+EPCR+CD48−CD150+ (E-SLAM) cells from E14.5 FL cells and adult BM are highly enriched for HSCs. Representative profiles of viable cells after being stained with antibodies to CD45-FITC, EPCR-PE, CD48-APC, and one of CD150-biotin/streptavidin-PE-TexasRed or CD150-PECy7 from E14.5 FL (A) and adult mouse BM (B). The E-SLAM fraction of cells represents approximately 0.02% of Ter119-depleted FL cells and approximately 0.004% of the adult BM population. The HSC purities in these suspensions were determined from monitoring mice injected with single E-SLAM cells for at least 16 weeks after transplantation. Plots were generated using FlowJo software from TreeStar (Ashland, OR).$

CD45⁺EPCR⁺CD48⁻CD150⁺ (E-SLAM) cells from E14.5 FL cells and adult BM are highly enriched for HSCs. Representative profiles of viable cells after being stained with antibodies to CD45-FITC, EPCR-PE, CD48-APC, and one of CD150-biotin/streptavidin-PE-TexasRed or CD150-PECy7 from E14.5 FL (A) and adult mouse BM (B). The E-SLAM fraction of cells represents approximately 0.02% of Ter119-depleted FL cells and approximately 0.004% of the adult BM population. The HSC purities in these suspensions were determined from monitoring mice injected with single E-SLAM cells for at least 16 weeks after transplantation. Plots were generated using FlowJo software from TreeStar (Ashland, OR).

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