Determining the frequencies of hematopoietic precursors in BALB and B6. The BMCs from old and young, DR- and AL-fed BALB mice were analyzed by 2 different flow criteria. (A) The gates used in the SP + Kit criterion, first gating for the Hoechst double low fluorescence side population,^37,38,44,45  and then for c-kit⁺. (B) The series of gates (lin⁻, c-Kit⁺, Sca-1⁻, CD34^±, CD135^±) used in the mKSL criterion³⁵  to determine LT-HSC, ST-HSC, and MPP subpopulations. (C) How the same mKSL criterion was used on B6 mice to determine the numbers of precursors.