RA signaling regulates hematopoietic potential and function of hemogenic endothelium during murine embryogenesis. (A) qPCR analysis of hematoendothelial gene expression in E9.5 Raldh2^−/− versus WT littermate yolk sacs. All data points were calculated as gene expression relative to endogenous β-actin expression (n = 3). (B) qPCR analysis of hematoendothelial gene expression in FACS-isolated subpopulations of E9.5 Raldh2^−/− and WT littermate yolk sac. All data points were calculated as gene expression relative to endogenous β-actin expression (n = 3). (C) Quantitative profile of hematopoietic potential of E9.5 Raldh2^−/− versus WT yolk sac. Data were calculated as the number of individual colonies generated per 1000 viable seeded cells plus or minus SEM (n ≥ 3).