ERK1/2 inhibition and sensitivity to PKC412 in FLT3_ITD627E cells. (A) Phosphorylation of ERK1/2 (T202/Y204) was analyzed by Western blotting in 32D_ITD and 32D_ITD627E cells after treatment for 2 hours with and without U0126. (B) Induction of apoptosis was assessed in 32D_ITD (■) and 32D_ITD627E () cells after treatment with PKC412 (10 nM), U0126 (10 μM), or a combination of both, for 24 hours (left panel) and 48 hours (right panel). Shown is 1 representative result of a total of 3.