May-Giemsa staining of cells sorted with anti–JAM-A antibody. (A) May-Giemsa staining of JAM-A⁺ (i) and JAM-A⁻ (ii) cells from BM of a 9-week-old mouse. Samples were examined using a Nikon Optiphot-2 microscope with a Nikon E plan 40×/0.65 numeric aperture objective and 10× eyepiece lens (Nikon, Tokyo, Japan). Images were acquired with an Evolution MP color camera (Media Cybernetics, Silver Spring, MD) and Image Pro Plus software (v4.5, Media Cybernetics). Arrows show progenitors. Scale bar = 10 μm. (B) The percentage of 4 phenotypically distinct groups in JAM-A⁺ and JAM-A⁻ cells from BM of a 9-week-old mouse: progenitors (immature blastic cells), mature myeloid, mature lymphoid and erythroid, or megakaryocyte lineage cells. Results from 2 independent experiments are shown. Error bars represent SD.