Figure 5
Figure 5. Donor GFP+ cells are not localized in the hair follicles of the skin. Immunofluorescence staining of the skin of the animals treated with GFP+CD150+CD48− BM infusion shows that GFP+ donor cells have localized outside of the hair follicles. Donor cells were visualized using anti-GFP antibody (green, top panel), and hair follicles were visualized using an anti–cytokeratin 5 antibody (red, middle panel). Donor cells and hair follicles are shown (vertical and horizontal arrows, respectively) on the image merged with blue DAPI nuclear stain (bottom panel).

Donor GFP+ cells are not localized in the hair follicles of the skin. Immunofluorescence staining of the skin of the animals treated with GFP+CD150+CD48 BM infusion shows that GFP+ donor cells have localized outside of the hair follicles. Donor cells were visualized using anti-GFP antibody (green, top panel), and hair follicles were visualized using an anti–cytokeratin 5 antibody (red, middle panel). Donor cells and hair follicles are shown (vertical and horizontal arrows, respectively) on the image merged with blue DAPI nuclear stain (bottom panel).

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