IL-7R is expressed on steady-state migDCs but not cDCs or pDCs. DC subsets from pLN, spleen, and skin were analyzed for IL-7Rα and γ_c-chain expression using flow cytometry. (A,B) Analysis of IL-7Rα expression on migDCs (CD11c⁺ MHCII^hi), CD8α⁺ and CD8α⁻ cDCs (CD11c⁺ MHCII^int), pDCs (CD11c^int PDCA-1⁺ CD45RA⁺), B cells (CD11c⁻ MHCII⁺) and T cells (CD8α⁺ CD11c⁻) in the pLN and spleen of WT (■) and IL-7Rα^−/− (▩) mice. (C) Analysis of IL-7Rα expression on DCs (7-AAD⁻ CD45⁺ MHCII⁺) isolated from the epidermis (top panels) or dermis (bottom panels) from WT (■) or IL-7Rα^−/− (▩) mice. These cells were also CD11c⁺ (not shown). (D) Expression of γ_c-chain was assessed in WT mice using an antibody against γ_c-chain (■) or an isotype control antibody (▩). Data are representative of 3 experiments.