Snrk-1 gene knockdown analysis in vitro. (A) RT-PCR for snrk-1 and actin genes in snrk-1 siRNA–transfected (500 ng) and control lacZ siRNA–transfected (500 ng) HUVECs. Lanes 7 through 9 show reduced snrk-1 transcripts compared with lanes 1 through 6. (B) In vitro KD studies show that there are fewer migratory ECs in snrk-1 siRNA–transfected HUVECs. The graph shows the effect of snrk-1 gene KD on migration of transfected HUVECs to serum-free medium (□) or 10% serum (■) for 5 hours at 37°C. All migration experiments have been conducted using HUVECs from passage numbers 2 through 4. The plotted data are pooled from 3 independent experiments and the error bars represent plus or minus SD. *P < .05 between snrk-1 siRNA and lacZ siRNA cells response to serum. (C) Graphic representation of the number of adherent ECs in snrk-1 siRNA–transfected, lacZ control siRNA–transfected, or untransfected HUVECs to an uncoated surface (□), laminin (), or fibronectin (■). Error bars represent SD.