BCL11A expression in human leukemia. (A) BCL11A expression in human chronic myelomonocytic leukemia and juvenile myelomonocytic leukemia was detected by RT-PCR. NC indicates water in place of cDNA as template for PCR. GAPDH was used as a loading control. The JMML samples are found to carry mutations in KRAS (no. 1) or PTPN11 (nos. 2-7), or wild type for both genes (no. 8). (B) Gene expression profiling in a large cohort of human acute myelogenous leukemia. Results using a set of 3 probes for BCL11A are displayed. Probe nos. 1, 2, and 3 indicate probe sets, 210347_s_at, 219497_s_at, and 219498_s_at, respectively. Clusters of AML showing increased level of BCL11A are noted (clusters 1 and 10).