CD41-GFP^low cells from the AGM seed the CHT and thymus. Fate of CD41-GFP^low AGM cells labeled at 48 hpf by laser-uncaging of caged rhodamine-dextran. All images are left lateral views, rostral to the left. (A) Rhodamine uncaging in 2 GFP^low AGM cells, led 17 hours later to (B) numerous rhodamine labeled cells in the caudal-most part of the CHT. (C-F) Rhodamine uncaging in 4 GFP^low cells in the AGM (C) led 12 hours later in the CHT to (D) a pair of double labeled cells (arrows and asterisks) linked by a tether (arrowheads), as sister cells usually are,⁴  and (E) several labeled cells including another a pair of sisters (arrow and asterisks); (F) By 80 hpf, 2 labeled cells were found in the thymus (arrows). Isv indicates intersomatic vessel. Scale bars, 20 μm.