Figure 5
Binding specificity of therapeutic monoclonal IgG1-F′2 IC to hFcγRs. Histograms show the binding of anti-FLAG mAb (thin line) or its isotype control (solid gray), and the binding of indicated monoclonal IgG1-F′2 IC to FLAG-tagged hFcγRs on CHO transfectants. Concentrations of monoclonal IgG and PE-F(ab′)2 anti–human Ig are indicated. Monoclonal IgG1 used here were produced in CHO cells. Solid gray histograms represent the binding of PE-F(ab′)2 anti–human Ig alone; 2 independent experiments gave similar results.
