Figure 2
Binding specificity of hFcγRs for IgG IC. Histograms show the binding of anti-FLAG mAb (thin line) or its isotype control (solid gray), and (A,B) the binding of IgG subclasses in complex with PE-F(ab′)2 anti–human F(ab′)2 to FLAG-tagged hFcγRs on CHO transfectants. Concentrations of human IgG and PE-F(ab′)2 anti–human F(ab′)2 are indicated. Solid gray histograms represent the binding of PE-F(ab′)2 anti–human F(ab′)2 alone; 4 independent experiments gave similar results. (C) Summary of IgG-F′2 IC binding abilities to hFcγRs using data from panels A and B.
