Figure 1
Binding specificity of hFcγRs for monomeric IgG. (A) Histograms show the binding of anti-FLAG mAb (thin line) or its isotype control (solid gray), and the binding of anti-FcγRI (CD64), anti-FcγRII (CD32), anti-FcγRIII (CD16), and anti-FcγRIIB/C (GB3) to FLAG-tagged hFcγRs on CHO transfectants. (B) Histograms show the binding of polyclonal human IgG subclasses to hFcγR-expressing transfectants using 10 μg/mL ultracentrifugated IgG and 15 μg/mL PE-F(ab′)2 anti–human F(ab′)2. Solid gray histograms represent the binding of PE-F(ab′)2 anti–human F(ab′)2 alone; 3 independent experiments gave identical results. (C) Summary of monomeric IgG binding ability.
