Cocultivation on OP9 stroma reveals that lineage potential of CD34⁺lin⁻ thymocytes is related to CD7 expression. Approximately 1500 cells from each population (CD34⁺lin⁻CD7⁻, CD34⁺lin⁻CD7^intCD10⁺, and CD34⁺lin⁻CD7^hi) were isolated by FACS, cocultured on OP9 stroma in FL, TPO, IL-7, and EPO and analyzed at 2 to 4 weeks for expression of erythroid (glycophorin A) and lymphoid (CD19, CD56) markers to evaluate the lineage potential of each subset. Shown is FACS analysis of one representative experiment from a total of 4 independent experiments, using different thymus samples.