The RGD sequence of rhAPC is critical for inhibition of neutrophil migration in vivo. (A) Neutrophil counts in BALF from LPS-treated mice. Mice were given rhAPC or RGE-APC (10 μg/mouse) or PBS 2 hours after LPS treatment. Results are expressed as mean (± SEM); n = 5 per group. (B) Survival curves for mice (C57BL/6, male, 6-8 weeks) challenged with an LD₉₀ of LPS; 200 μg of the RGD peptide or the control (RAD) peptide was given via intravenous injection before receiving 37 to 40 mg/kg LPS intraperitoneally (n = 15-25 per group). The statistical significance of mortality was determined by the Kaplan-Meier log-rank test.