Inverse relation between p73 and Itch in primary CLL consequent to deacetylase inhibition. (A) Primary leukemia cells that were normal or del17p were treated with 10 nM LBH589 for 36 hours. Changes in acetylation levels of histone H3, expression levels of p73, Itch, and GAPDH were measured. (B,C) Quantification and inverse relation between p73 and Itch expression in response to LBH589 in 33 primary leukemia samples. The Pearson correlation between paired samples was conducted (P < .05, 2-tailed P value, Pearson correlation r = −0.4). (D) K562 cells were transfected with short interfering RNA that were scrambled or against Itch for 48 hours, followed by immunoblotting for Itch, p73, and GAPDH.