The Liar sequence. (A) The full-length amino acid sequence of Liar, illustrating the P-loop (blue letters), NLS (red letters), NES (green letters), potential serine phosphorylation sites (triangle), and the ankyrin repeats (boxed). (B) The genomic organization of Liar showing the relative size/location of the introns and exons (E1-8). (C) Representation of Liar domain organization (P-loop, dark blue; NLS, red; ankyrin repeats, light blue; NES, green); amino acids at the domain boundaries are numbered. (D) Alignment of the 4 Liar ankyrin repeats with the ankyrin consensus, illustrating the degree of identity (red) and homology (yellow). *Residues highly conserved among all ankyrin repeats. (E) Western blot analysis of Liar and Lyn protein expression in hemopoietic cell lines, quantitated relative to Erk2 levels (E, erythroid; M monocyte; Mt, mast cell; B, B cell; T, T cell). (E) In situ hybridization showing expression of Liar mRNA during embryonic development. Mouse embryos at day E10 were probed with sense and antisense probes. Intense Liar expression was detected in the fore (F) and hind (H) limb buds, the maxillary process (M), the branchial arches (B), and the gastrointestinal tract (GI).