Rho A is localized to some extent at the cleavage furrow in endomitotic MK. Cells were stained for tubulin (α and β, green) and RhoA (red). (A) RhoA localization during anaphase. In dipolar mitotic/endomitotic MK, RhoA was concentrated around the midzone cortex (i,ii). In polyploid endomitotic MK, RhoA could not be detected in the midzone (iii,iv). (B) RhoA localization during telophase. In dipolar mitotic/endomitotic MK, RhoA was concentrated in the cleavage furrow (i,ii). In polyploid endomitotic MK, RhoA was only detected in the deepest ingressions of the cleavage furrow (iii,iv, arrows 1,2). (C) RhoA localization at the end of telophase. RhoA was localized at all sides of the cleavage furrow in dipolar mitotic/endomitotic MK (iii,iv). In multipolar endomitosis, RhoA was accumulated at only one part of the cleavage furrow and at a zone corresponding to a local ingression (indicated by arrow, i,ii).