Western blot of constructs 1 to 4 and platelet lysate (Pl) using monoclonals AP3, AP5, and anti-V5 for detection. Constructs 1 to 4 migrated as expected on the basis of their predicted molecular weights and reacted with the appropriate probes. As expected, construct 4 containing mutations A50S, R62Q, and D66A was not recognized by monoclonal AP3, and platelet GPIIIa (Pl) was not recognized by anti-V5.