Liver sections. Nude mice were treated intravenously with PBS, 2 mg/kg of HA22, or 20 mg/kg of HA22-LR. After 30 hours, livers were removed, fixed in formalin, sectioned, and stained with hematoxylin and eosin. Panel A (original magnification ×400) and panel D (original magnification ×200) show a representative section of the PBS-treated mouse. Panel B (original magnification ×400) and panel E (original magnification ×200) show a representative section of the HA22-treated mouse. There are many individual necrotic hepatocytes in various stages of cell death as well as the loss of hepatocytes and replacement with blood in sinusoids. Panel C (original magnification ×400) and panel F (original magnification ×200) show a representative section of the HA22-LR-treated mouse. Slides were viewed with an Olympus BX41 microscope. Images were acquired using an Olympus DP41 camera with its own Olympus DP Controller software. Photoshop CS3 was used to produce the composite.