Dendritic morphology of dendritic epidermal T cells is influenced by integrin α_E(CD103) in vivo. (A) Epidermal sheets of wildtype and integrin α_E(CD103)-deficient mice on the C57BL/6 genetic background (littermates) were prepared and DETCs were visualized using an mAb directed against CD3 as outlined in “Isolation of epidermal sheets and immunohistochemistry.” The scale bar indicates 20 μm. (B) The surface area spanned by DETC dendrites in wild-type (left bar) and integrin α_E-deficient littermates (right bar) was analyzed using ImageJ software. The values shown represent data from 50 DETCs that were analyzed from 3 mice of each genotype.