Gene cross-validation in human primary follicular B-cell lymphomas. Correlation in gene expression profile of MCL1-positive primary follicular B-cell lymphomas. Gene expression profiling was performed in 33 cases of follicular B-cell lymphomas using CNIO Oncochip 12K microarrays (ArrayExpress accession number A-MEXP-261). Total RNA was isolated in 2 steps using Trizol (Invitrogen) followed by RNeasy (Qiagen, Valencia, CA) purification. Amplification of RNA was performed from 4 μg of total RNA using the Superscript System for cDNA synthesis (Invitrogen) and the T7 Megascript in vitro transcription kit (Ambion, Austin, TX). Amplified RNA (2.5 μg) was directly labeled with Cy5-conjugated dUTP. RNA (2.5 μg) from the Universal Human Reference RNA (Stratagene) was labeled with Cy3-conjugated dUTP as reference. Raw data (Cy5/Cy3 ratios) for 33 cases of MCL1-positive human follicular B-cell lymphoma are shown.