Effects of Ik6 overexpression on GPA⁺ and CD13⁺ sorted cells that were subsequently cultured in erythroid or myeloid conditions. (A) CD34⁺ cells from CB were maintained in erythroid condition for 10 days. On day 3, cells were transduced with EF1 GFP and EF1 IK6 vectors. On day 10, untransduced and transduced cells were stained with CD13-PE and GPA-PC5 antibodies. CD13⁺/GPA⁻ (CD13⁺ sorted cells) and CD13⁻/GPA⁺ (GPA⁺ sorted cells) were sorted by FACS. These 2 populations were replated in erythroid or myeloid conditions for 7 days, when subcultured cells were harvested, and living cells were counted by trypan blue exclusion and phenotyped for different cell-surface markers (erythroid marker, GPA; myeloid markers, CD14, CD13, and CD33). (B) Phenotype of erythroid cultured CD13⁺-sorted cells for CTRL (solid histogram), EF1 GFP (dashed line), and EF1 IK6 (bold line) conditions. (C) Phenotype of myeloid cultured GPA⁺-sorted cells for EF1 GFP (dashed line) and EF1 IK6 (bold line) conditions.