Effects of Ik6 overexpression on erythroid cell phenotypes. On days 10, 13, 17, and 20, CB untransduced and transduced cells were harvested and were analyzed for different cell-surface markers (erythroid markers: CD36, CD71, GPA and CD111; progenitor markers: CD117 [c-kit], CD34; human hematopoietic marker: CD45; and myeloid markers: CD33 and CD13) by flow cytometry. On day 20, CD36⁺, CD71⁺, GPA⁺, and CD111⁺ cell percentages (A) and numbers (B) are shown (mean ± SEM for 7 independent experiments). On day 20, percentages (C) and numbers (D) of CD117⁺, CD34⁺, CD45⁺, CD33⁺, and CD13⁺ cells are shown (means ± SEM for 4 to 7 independent experiments). The unchanged results on days 10 and 13 are shown in Table S1. *P < .05 using the Wilcoxon test compared with CTRL and EF1 GFP conditions; n.s. indicates not significant. Representative dot plots (E) show typical expression of CD13 and GPA, CD13 and CD33, CD13 and CD45, and CD13 and CD117 on day 20 for transduced and untransduced cell populations. Percentage in the quadrants is the percentage of cells present in the defined gate.