Cytokine flow cytometry results at each time point. PBMCs collected before and after TRICOM-CEA vaccination were analyzed for intracellular production of IFNγ in response to rF-CEA(6D)-TRICOM using flow cytometry. The data are presented as the mean percentage of CD4⁺ or CD8⁺CD69⁺IFNγ⁺ T cells for patients completing 4 injections for each cohort. Arrows indicate time of denileukin diftitox administration.