Figure 3
![Figure 3. Pulse-chase analysis of PZ and FX secretion. HEK 293 cells transiently transfected with expression vectors were divided into 5 dishes and were labeled with a 50 μCi [35S]protein labeling mixture for 1 hour. The chase was then initiated by changing the medium containing Vit.K, and the samples were incubated for 1, 2, 4, and 8 hours. PZ (A) or FX (B) in the medium (●) and in the cell lysates (○) were collected by immunoprecipitation using an anti-PZ or anti-FX antibody. Their intensity under fluorography was calculated by setting the signal in the cell lysates at chase-time 0 to 100%. The data shown are the mean (± SD) for 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/16/10.1182_blood-2008-07-171884/7/zh80170934010003.jpeg?Expires=1724237379&Signature=xUBvKipRMvBPnyARjyNZTnQ5h8jrAw6mCS0oWk3JdOinFU-iIaIWE23p824UpNgYLK1kH4I1NfEEjdSAvt2kSwmYrRp4fLp7rLN42NEWxPurMCYGOShN0FsqLzcDw~882CpogMXBcphwPqUhUlNz7cpvf243NtrjUFiwpE92zy7wo3B0UgNH8YivW0pr8IMAULRepbUo3Z2cQ0UB4dRx92iZJJ5ftGkvOKtXJ4AFFzLFIq75aV9F1RRdFi7JZmXZXHuzVwIqSUZPTi-3OQhAFYl1jhyEmc3MzaikwbAzIU-wrnwhaMW0IFuVSP1cE9ZoaairvBa2tcRUAdBm0BfA1Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of PZ and FX secretion. HEK 293 cells transiently transfected with expression vectors were divided into 5 dishes and were labeled with a 50 μCi [35S]protein labeling mixture for 1 hour. The chase was then initiated by changing the medium containing Vit.K, and the samples were incubated for 1, 2, 4, and 8 hours. PZ (A) or FX (B) in the medium (●) and in the cell lysates (○) were collected by immunoprecipitation using an anti-PZ or anti-FX antibody. Their intensity under fluorography was calculated by setting the signal in the cell lysates at chase-time 0 to 100%. The data shown are the mean (± SD) for 3 independent experiments.
