UA does not prevent increased T-cell immunity induced by other means. (A) The peripheral levels of Tregs (CD4⁺CD25⁺Foxp3⁺ or CD8⁺CD25⁺Foxp3⁺) in animals injected with dying tumor cells alone (T) or with tumor cells with UA (T + UA). Each bar is the mean (± SEM; *P < .05) of 3 mice. (B) Levels of neu peptide tetramer⁺ CD8⁺CD62L^lo T cells (ie, effector or effector memory) in control animals, or animals injected with tumor cells (T) with or without UA (UA) or ONTAK (On). Each bar is the mean (± SEM) of 3 to 4 mice. (C) The tumor growth rates in mice pretreated with tumor cells alone and tumor cells with or without UA, ONTAK, or both. Each data point is the mean (± SEM) of 3 to 5 mice. *P < .05 compared with all other groups.