D6 colocalized with markers of rapid and slow recycling pathways. Confocal images of immunofluorescence-stained CHO-K1/D6 cells. Panels show representative experiments of D6 staining with vesicle markers EEA1 (A), Rab4 (B), Rab11 (C), Tf (D), syntaxin 6 (E), furin (F), VAMP2 (G), IRAP (H), and LAMP1 (I). In the first column, the nuclear staining (DAPI, light blue) is merged to the DIC image; D6 and vesicle marker expression alone are shown in second and third column, respectively. The fourth column represents the double staining of D6 (red) and vesicle markers (green). Inserts represent magnifications of the boxed area in the double-staining images.