Multimeric composition of plasma mVWF after hydrodynamic gene delivery. VWF^−/− mice were injected with 50 μg of WT pLIVE-mVwf (WT) or pLIVE-mVwf mutated in the RGD sequence (RGG), in the collagen-binding site (Coll mutant), or in the GPIb-binding site (GPIb mutant). Plasma was collected 72 hours later, and analysis of plasma samples was performed by SDS/1% agarose gel electrophoresis. NMP indicates normal mouse plasma.