Magnitude and antigenic repertoire of HHV-8–specific T cells as assessed by ELISpot IFN assays in patients with MCD (n = 12) and HIV-coinfected AC (n = 12). HHV-8 ELISpot IFN-γ assay used 56 HHV-8–specific peptides, including 4 previously identified epitopes in lytic GpB, GpH, and Gp35/37, 9 optimal peptides predicted in latent LANA-1, and 43 overlapping peptides covering latent K12, and exons 1-3 in latent K15. Results are given as frequencies of SFC/10⁶ PBMC. (A) Individual magnitude of HHV-8–specific T-cell responses in MCD and AC patients. The sum of positive responses (> 50 SFC/10⁶ PBMC) was calculated individually. Solid symbols represent patients with positive responses and empty symbols patients with negative responses. The Mann-Whitney nonparametric test was used to compare groups. (B) No correlation between individual sum of each person's HHV-8–positive T-cell responses and CD4 cell counts in peripheral blood (cells/mm³), calculated with the Spearman nonparametric test in all 24 patients. (C) No correlation between the sum of each person's HHV-8–positive T-cell responses and HHV-8 viral load in PBMC (copies/150 000 PBMC), calculated with the Spearman nonparametric test in 20 patients (4 viral load missing). VL indicates viral load; cp, copies. (D) Antigenic repertoire of HHV-8–specific T-cell responses. Number of positive responses against each protein is shown for MCD and AC group.