Knockdown of endogenous TF in monocytes reduces TF activity in granulocytes. MNC-TF activity was dramatically reduced when si-TF–nucleofected monocytes, but not granulocytes, were reintroduced into whole blood (A). Reintroduction of si-TF–nucleofected monocytes led to a profound reduction in LPS/PMA-stimulated TF activity in granulocytes (B). Data are presented as percentage of si-CTR–nucleofected samples; error bars are SEM. *P < .05 compared with si-CTR monocytes.