CD40-mediated up-regulation of BCL6 target genes is reversible. Ramos cells were exposed to CD40L for 1 hour and then split into 3 fractions. The first fraction was immediately lysed (), the second was washed to remove the CD40L and then cultured for an additional 5 hours (▨), and the third fraction remained in CD40L containing media for another 5 hours (■). QPCR was performed in untreated cells and all 3 fractions to detect mRNA abundance of BCL6, ATR, CCL3, and CD23b. The mRNA levels of these genes were first normalized to GAPDH mRNA levels and expressed as fold increase relative to untreated cells. The experiment was performed in triplicate. Error bars represent SEM.