Effect of dasatinib and imatinib on BCR-ABL function. Effect of dasatinib and imatinib on pCrkL expression (A) in mock-transfected KCL22 cells ( bars) and KCL22 cells with high hOCT1 expression (■ bars). Data are expressed as relative to untreated cells. Dasatinib produced a significant decrease in pCrkL expression for both cell lines, P = .011 and P = .003 in mock and high hOCT1-expressing cells, respectively. Imatinib produced a significant decrease, with P = .001 only in high hOCT1-expressing cells. * denotes a significant effect. pCrkL suppression by imatinib was greater in high hOCT1-expressing cells than in mock-transfected cells (P = .026). Data represent mean plus or minus SEM from 5 experiments. Examples of the corresponding p-CrkL flow histograms for mock (B) and hOCT1-expressing KCL22 cells (C) are also presented.