Transfection analysis of zAbcg2. COS-7 cells were transiently transfected with the pcDNA3 vectors containing zAbcg2a, zAbcg2c, or ginbuna CD8α (gCD8α) as a control, and these cells, zAbcg2a/COS-7, zAbcg2c/COS-7, and gCD8α/COS-7, were stained with Hoechst 33342 (Hoechst) and analyzed by flow cytometry. The zAbcg2a/COS-7 (A), zAbcg2c/COS-7 (B), and gCD8α/COS-7 (C) cells are displayed in Hoechst red versus Hoechst blue plot. (D) Hoechst fluorescence of zAbcg2a/COS-7 stained with Hoechst in the presence of 250 μM verapamil is shown. (E) The results of RT-PCR analysis of zAbcg2a in SP COS-7 and MP COS-7 cells from zAbcg2a/COS-7 cells are shown. The β-actin from vervet monkey (Cercopithecus aethiops) was used as an internal control. NC indicates negative control.