Flow cytometric analysis of zebrafish kidney hematopoietic cells. Hematopoietic cells from zebrafish body kidney were stained with Hoechst 33342 (Hoechst) and analyzed by flow cytometry. (A) Hoechst fluorescence (Hoechst blue vs Hoechst red) of kidney hematopoietic cells is shown. Gated region indicates SP population. (B) Hoechst fluorescence of kidney hematopoietic cells that were stained with Hoechst in the presence of 250 μM verapamil is shown. (C) Scatter profile of SP cells is shown. Lymphoid indicates the FS^low, SS^low fraction; myeloid, the FS^high fraction. (D) Morphologic analyses of lymphoid-SP (Lym-SP) (i) and myeloid-SP (Mye-SP) (ii) are shown (May-Grünwald Giemsa, scale bar = 5 μm).