G₁ phase changes from unstimulated (US) and stimulated (S) B-CLL cells. G₁ phase changes (mean percentages ± SD) from unstimulated (US) and stimulated (S) B-CLL cells were evaluated using the AO technique after 48 hours of culture in (A) IgV_H unmutated and (B) IgV_H mutated samples. Representative experiment obtained from an IgV_H unmutated patient is shown in the box.