Figure 2
IL-7–induced proliferation of adult naive CD4+ T cells is restricted to the CD31+ subset. (A) Representative dot-plots of CD31 and Ki67 flow cytometry analysis after 7-day culture in the presence of IL-7 of purified CD31+ and CD31− naive CD4+ T-cell subsets from adult peripheral blood, for an IL-7 “nonresponder” (top panel), an IL-7 “responder” (middle panel), and CB (bottom panel). Cells were successively gated on a viable lymphogate, CD3+, CD4+, and CD45RA+. (B) CD31 MFI was assessed within the purified CD31+ naive subset further gated on Ki67+ or Ki67− cells after 7-day culture with IL-7. Three adults and 4 CB samples were studied. (C) Representative dot-plot illustrating CD31 expression plotted against CFSE labeling of CB CD4+CD45RA+ T cells cultured with IL-7 for 7 days. CD31+ cells were further gated according to the number of cell divisions, and bars show CD31 MFI from 4 experiments. (D) Ex vivo analysis of IL-7Rα MFI on freshly isolated mononuclear cells from adult and CB samples sequentially gated on CD3+, CD4+, CD45RA+, and CD31+ or CD31− lymphocytes. Each symbol represents one individual. Bars represent mean plus or minus SEM. Data were compared using paired or unpaired t test as appropriate and significant P values are shown.

IL-7–induced proliferation of adult naive CD4+ T cells is restricted to the CD31+ subset. (A) Representative dot-plots of CD31 and Ki67 flow cytometry analysis after 7-day culture in the presence of IL-7 of purified CD31+ and CD31 naive CD4+ T-cell subsets from adult peripheral blood, for an IL-7 “nonresponder” (top panel), an IL-7 “responder” (middle panel), and CB (bottom panel). Cells were successively gated on a viable lymphogate, CD3+, CD4+, and CD45RA+. (B) CD31 MFI was assessed within the purified CD31+ naive subset further gated on Ki67+ or Ki67 cells after 7-day culture with IL-7. Three adults and 4 CB samples were studied. (C) Representative dot-plot illustrating CD31 expression plotted against CFSE labeling of CB CD4+CD45RA+ T cells cultured with IL-7 for 7 days. CD31+ cells were further gated according to the number of cell divisions, and bars show CD31 MFI from 4 experiments. (D) Ex vivo analysis of IL-7Rα MFI on freshly isolated mononuclear cells from adult and CB samples sequentially gated on CD3+, CD4+, CD45RA+, and CD31+ or CD31 lymphocytes. Each symbol represents one individual. Bars represent mean plus or minus SEM. Data were compared using paired or unpaired t test as appropriate and significant P values are shown.

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