Integration site analyses in the bone marrow of primary and quaternary recipients infused with LV-transduced Lin⁻Sca1⁺ samples. (A) Representative LAM-PCR analysis of BM samples from primary and quaternary recipients that received a transplant of the chimeric BM graft corresponding to experiment no. 2. M indicates size markers; 5′IC, internal control band amplified in LV-transduced samples. (B) Distribution of LV integrations in genes, or in repetitive sequences (LINES, SINES, LTR, and RNA) of the mouse genome. (C) Distance of 24 LV integration sites to the transcription start sites of targeted genes in the mouse genome. The distance is represented by intervals of plus or minus 10 Kb from 0 to 100 Kb around TSSs, or by intervals of plus or minus 50 Kb for distances higher than 100 Kb.