Plasma cytokine concentrations in C57BL/6 mice at 2, 7, and 20 hours after transfusion. WT C57BL/6 mice were passively immunized with 2 mg 10F7 anti-hGPA IgG1 Mab, 100 μg 6A7 anti-hGPA IgG1 Mab, or 2 mg anti-HEL (irrelevant, isotype-matched control) IgG1 Mab. Mice were transfused with DiO-labeled WT C57BL/6 RBCs (as a negative, internal control) and with either DiI-labeled incompatible hGPA-Tg RBCs (data shown) or compatible WT FVB/NJ RBCs (data not shown because all of these results are very similar to the anti-HEL controls). IL-6 (A), TNF-α (B), MCP-1 (C), IFN-γ (D), IL-10 (E), and IL-12p70 (F) concentrations were measured in plasma, as described in “Cytokine measurements” (note that the scale on the y-axis varies from graph to graph and the absence of bars represents results below the limit of detection of the assay). Five mice were evaluated at each time point and the mean plus or minus 1 SD is provided. Data shown are from 1 representative experiment (from a total of 4 replicates). *P < .05 compared with the anti-HEL controls.