VWF-CFP type 2N variants induce the formation of storage organelles that recruit VWF propeptide and P-selectin in HEK293 cells. HEK293 cells were transfected with VWF-CFP type 2N variants alone (A) or in combination with P-selectin (B). Cells were stained for VWF propeptide (in green, A) using monoclonal antibody CLB-Pro17 and for P-selectin (in green, B) using rabbit polyclonal anti–human CD62P antibody. VWF-CFP type 2N variants are depicted in red (A,B). Shown is the merge of double-fluorescent detection of representative 3-dimensional projections (Z-stacks 0.4 μm). Regions of colocalization are shown in yellow. The scale bar represents 10 μm.