Inhibition of PI3K suppresses CXCR3 induction following in vitro T-cell activation. T cells from C57BL/6 mice were stimulated with anti-CD3/anti-CD28 antibodies for 48 hours and then rested with different concentrations of PI3K inhibitors: (A) wortmannin (10 nM, 100 nM, or 3 μM) or (B) LY294002 (2.5 μM, 5 μM, or 100 μM). Following 24-hour incubation with inhibitors, expression of CXCR3 on these cells was analyzed by flow cytometry. Isotype control–stained cells are represented by hollow, broken lines. Data are representative from 1 experiment of 3 independent experiments with similar results.