The PI3K signaling pathway is activated by LPS in BMDCs. BMDCs were cultured overnight and then pretreated with either 100 nM wortmannin or 10 ng/mL rapamycin for 20 minutes or left untreated before being stimulated with 1 μg/mL LPS for the indicated times. Cell lysates were analyzed for phospho-Akt, phospho-GSK3α/β, phospho-TSC2, p70S6K, phospho-p38, phospho-ERK, phospho-JNK, IκBα, GSK3α/β, and ERK2 by Western blotting. Four dots added between the 10-minute and 30-minute lanes of p70S6K samples indicate the migration positions of hyperphosphorylated p70S6K caused by multiple phosphorylation events, which are represented as a through d on the left side (Figure S1).