Stimulation bypassing the TCR complex can overcome the effects of SFK inhibition. (A) PBMCs were stimulated with OKT3 in the presence or absence of 10 nM dasatinib for 20 hours, and a portion were analyzed by flow cytometry for CD69 (top row). The same groups were then stimulated with PMA (50 ng/mL) for an additional 20 hours and T cells analyzed again for CD69 expression (bottom row). (B) PBMCs were stimulated with PHA or CD3/CD28 beads for 72 hours and then stained with SYTOX green and annexin V and analyzed by flow cytometry.