Figure 4
![Figure 4. APO866-mediated cell death involves autophagy. ML-2, Namalwa, Jurkat, or primary AML cells pretreated with either inhibitors of autophagy (wortmannin [WMN], LY294002, 3-methyladenine [3-MA], or bafilomycin A1 [BAFA]) or inhibitor of translation cycloheximide (CHX) were exposed to 10 nM APO866 for either 72 hours (A-D) or 96 hours (E-H). APO866-induced cell death was assessed as described in Figure 1. Percent cell death induced by drug = [(S-C) / (100-C)] × 100; where S = treated sample cell death and C = untreated sample cell death. Data are derived from at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/14/10.1182_blood-2008-08-173369/4/zh80170934250004.jpeg?Expires=1724259347&Signature=BT95I2fs1TqxDqydbiYuj6zI9cDMh5lJPZM7Pa8gqv5~hC5xnaL3NYzW4qJJkZJUhtnxgzIrcV7QvHVIJLb0JtH~ecreu9RieiP6B3gIBqOBWs-ju~kKzXyVapqPIDLRx3-VGcdQKCTIRi3UUUg0KrIfrAlhDnetbXkrcSSpek3uLb4b3q0D2qaGrwbPTgbavPY4hgkJwbj1~HRKHftznZxO3y9MomId-v7tlLPd0G5cmhUuEeCf5tbyu~SwVLKpgYCXPGlm8F~OQNi-RJ7R1g~DbWzjpews1MCPRz2veXHH98zxRVrPw5afSo6CBkpSoLNmykFHZZ-jp6Ysvse4cQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
APO866-mediated cell death involves autophagy. ML-2, Namalwa, Jurkat, or primary AML cells pretreated with either inhibitors of autophagy (wortmannin [WMN], LY294002, 3-methyladenine [3-MA], or bafilomycin A1 [BAFA]) or inhibitor of translation cycloheximide (CHX) were exposed to 10 nM APO866 for either 72 hours (A-D) or 96 hours (E-H). APO866-induced cell death was assessed as described in Figure 1. Percent cell death induced by drug = [(S-C) / (100-C)] × 100; where S = treated sample cell death and C = untreated sample cell death. Data are derived from at least 3 independent experiments.
