Signaling BMDCs via NK1R prevents apoptosis through activation of the PI3K-Akt cascade. (A) Bar diagram representing the effect of intracellular pathway blockade on the antiapoptotic effect exerted by SarSP on CD11c⁺ BMDCs. Before culture with or without SarSP, BMDCs were left untreated (control) or incubated with the cell-permeable inhibitors LY294002 (PI3K), PD98059 (ERK), SN-50 (NFκB), or JNK inhibitor II (JNK-AP1). ***P < .001. (B) FACS analysis showing the level of pAktSer473 expression by CD11c⁺ BMDCs cultured in the presence of SarSP (gray empty histogram) or medium alone (gray filled histogram). (C) Comparative analysis of the effects of LY294002 and Akt inhibitor X (pAktSer473 inhibitor) on the antiapoptotic effect of SarSP on BMDCs. ***P < .001. (D) FACS analysis illustrating the level of Bcl-2, pBadSer136, and active caspase 3 expression by CD11c⁺ BMDCs cultured in the presence of SarSP (black empty histogram), GM and IL-4 (gray empty histogram), or medium alone (gray filled histogram). (A, C) Means (± SD) of 4 independent experiments are shown. (B, D) Data are representative of 4 independent experiments. Numbers in histograms indicate percentage of CD11c⁺ BMDCs.