HPAEC profile analysis of IgG1-Fc. Trifluoroacetic (TFA) hydrolysis resulted in complete degradation of the N-glycan within the separate Fc fragments to compositional monosaccharides where N-acetylglucosamine (GlcNac) was released as glucosamine. Compositional monosaccharide patterns were compared with a standard monosaccharide profile composed of fucose, glucosamine, galactose, glucose, and mannose, respectively. Fuc indicates fucose; GlcN, glucosamine; Gal, galactose; Glc, glucose; Man, mannose; GP-2, standard glycopeptide; GA, aglycosylated chimeric anti-CD137 mAb; GG, glycosylated chimeric anti-CD137 mAb; CTM, cetuximab; and HuIgG1, polyclonal human IgG1.