Functional DNA-binding and transactivation domains of Runx2 are required to induce AML in cooperation with Cbfβ-SMMHC. (A) Depiction of point mutants R217Q (in DNA-binding RHD), Y418A, and HTY (within NMTS) used in this study. (B) Western blot analysis showing the expression of wild-type Runx2 (lane 1), R217Q (lane 2), Y418A (lane 3), and HTY (lane 4) from the constructs used in this study. (C) Kaplan-Meier survival curve of mice transplanted with cells expressing Cbfβ-SMMHC and wild-type Runx2 (thick solid line, n = 22), R217Q (thin dashed line, n = 7), Y418A (gray dashed line, n = 8), HTY (thick dashed line, n = 8), or mock (thin solid line, n = 24). Analysis of statistical significance was performed using the log-rank test.