Promoter analysis of the human c-FLIP gene. (A) The gene structure of the human c-FLIP gene (CFLAR), which is located on chromosome 2, is shown. CFLAR comprises 14 exons spanning approximately 52 kb with exon 3 containing the translational start codon. The regions coding for the first DED (1DED), the second DED (2DED), and the caspase-like domain of the long isoform are indicated. The 2 potential promoter regions are in front of exon 1 (P1) and exon 3 (P2), respectively, and are represented by —. (B) Sequence of c-FLIP promoter P1. The sequence of chromosomal region 201,688,637 to 201,689,236 of human chromosome 2 is shown. Promoter P1 is shaded in gray. The core nucleotides of 3 potential NFAT binding sites (GGAAA) are marked in bold, and a potential NF-κB site (aaaGGActc) is in italics. The oligonucleotide sequences used as probes for EMSA analyses are underlined.