Eosinophil chemotaxis and activation mediated by M bovis BCG. (A) Eosinophil migration in response to different BCG/eosinophil ratios. Error bars represent plus or minus SEM from 5 independent experiments. (B) ROS generation and EPO release by eosinophils in cell supernatants. Results are expressed as ΔROS or ΔEPO cps (counts per second) values (values from medium stimulation are subtracted from values obtained with each ratio). (C) After 18 hours of incubation, ECP concentrations in the cell-free supernatants were measured. Data are expressed as means plus or minus SEM from the 3 independent experiments with different eosinophil donors. (D) Eosinophils were stimulated for 18 hours at different BCG/ eosinophil ratios. TNF-α levels were quantified in the culture supernatants by ELISA. Results are represented as the mean of 3 independent experiments plus or minus SEM. *P < .05; **P < .01.